人工材料表面形态对转化人胚肌腱细胞黏附特性的影响

Effects of Surface Pattern of Polymers on Adhesion Characteristics of Transformed Human Embryonic Tendon Cells

利用微吸管实验技术这一细胞力学手段研究人工材料表面形态对转化人胚肌腱细胞黏附特性的影响。结果显示 :在不同形态聚合物表面 ,转化人胚肌腱细胞具有不同的黏附特性 ,细胞与多孔膜的黏附比与无孔膜和纤维的黏附大 ;细胞与多孔膜的黏附力随多孔膜孔径增大而增加 ,当孔径较大时 (15 0～ 5 0 0μm) ,细胞与多孔膜的黏附力明显增加 (P<0 .0 5 ) ;转化人胚肌腱细胞与纤维的黏附力随纤维直径增大而增加 ,但无显著性差异 (P>0 .0 5 )。提示 ,选择特定孔径的聚合物泡沫或特定直径的聚合物纤维制成组织工程化肌腱支架 。

Effects of surface pattern of polymers on adhesion characteristics of transformed human embryonic tendon cells (THETCs) have been examined by using a micropipette aspiration technique, which is a cellular mechanical method. The results showed that the adhesion characteristics of THETCs depend on the surface pattern of polymers, and the adhesion force of THETCs to porous films is higher than that to non-porous films and fibers. The adhesion force bears relationships with the aperture of porous films and has a stronger dependence on the large aperture (150 [similar to] 500 μm) than on the small aperture ( less than or equal 150 μm) of porous films. The adhesion force of THETCs to fibers is increased slightly with the diameter of fibers(P>0.05). These demonstrat that the tissue-engineered tendon scaffolds made of porous polymer foam with specially designated aperture, or of polymer fiber with specially designated diameter, may serve as a good attachment substrate for THETCs.