大鼠角朊细胞导电聚吡咯膜原代培养法的建立

Rat Keratinocyte Primary Cultures Based on Conductive Polypyrrole Primary Cell Culture Technique

采用正交实验设计方法 ,对影响大鼠角朊细胞生长的细胞培养液中四种主要组成成分及导电聚吡咯膜促细胞生长条件进行了研究。结果表明 ,每 10 0 0 m l DMEM中胎牛血清、NEAA、胰岛素、氢化可的松的含量分别为10 %、1%、6 ml、10 m g时 ,对角朊细胞的培养效果较佳 ;而浓度为 0 .1M的吡咯单体在电流为 10 m A、通电 10 0 s条件下制备的聚吡咯膜 ,在电刺激电压 10 0 m V,刺激时间为 2 h的条件下对角朊细胞生长作用最显著 ;且在此培养条件下检测的细胞数是常规培养的 1.

Orthogonal experimental design has been applied to study the suitable cell culture medium and condition of conductive polypyrrole on rat keratinocyte cell cultures. The results showed that one thousand milliliters of complete minimum essential medium (CMEM) which contained 900 ml minimum essential medium (MEM), 10% fetal bovine serum (FBS), 1% NEAA, 6 ml insulin and 10 mg hydrocortisone was fit for keratinocyte cell culture. And the conductive polypyrrole obtained with 10 mA current density for 100 second switching times, given 100 mV electrical stimulation voltage for 2 hours, would stimulate the growth of rat keratinocyte cell in vitro cultures. The cell density under culture conditions described above was 1.30 times as high as that under routine culture conditions.