摘除眼柄诱导斑节对虾成熟产卵的研究

THE STUDY OF EYE STALK EXCISION OF MONODON TO INDUCE ITS MATURITY AND SPAWNING

本实验结果表明,摘除斑节对虾(Penaeus monodon Fabficius)左或右侧眼柄,可以诱导卵巢发育成熟产卵,且成活率高,亦不影响正常摄食和交配活动。不摘除眼柄和摘除两侧眼柄,迄今尚未见到卵巢发育之虾。 实验还表明:选择雌虾体长210mm、体重140g以上,在水温27—3l℃,盐度30—35‰,pH值7.2—8.5,弱光(光照度100—500Ix),光质为蓝或绿光,溶解氧4mg/L以上,外界环境尽量避免干扰,微循环水流动,饵料以贝类,日投喂量10%的条件下培养,催熟效果好。 本实验也为在对虾属中进行种间杂交培育优良品种提供了依据。

The excision of Monodon's eye stalk (Left or right eye) does induce its maturity and spawning. It also leads to high survial rate without affecting its normal eating and mating activity. No adverse effect was found if their eye stalk (right or left) or eyes are takan off.Excision of Monodon's eye stalk plays an important part in inducing its maturity and spawning. Best results were obtained in the following experimental conditions.sex: femaleLength: above 210mmWeight: above 140gWater tempreture: 27-31 degree centigrataSalinity: 30-35‰pH: 7.2-8.5Illumination: 100-500 luxes(blue or green)Dissolved oxyen: above 4mg/LExternal environment: without interference and, with slow circulated runningwaerFeed: shellfish, 10% each dayThe prawn will reach the peak of maturity within 15 to 35 days after eye stalk excision. This experiment showed that the rate of acceleration of ripening is 57.4 % (highest can be 1(30%); average fertilization rate is 59% (highest is 88.9%); number of eggs produced generally depends on prawn's size (the bigger the prawn, the larger amount of eggs it will lay). Usually 2 to 4 hundred thousand eggs 10 to 30% less than the natural mature prawn; The average hatch rate is 72.3%, maximum 97.1%. Prawns with excision of eye stalk may spawn 2 to 3 times the maximun 5 times, but moult easily so that they lose spermalophores when environment is not stable.