不同药物治疗创伤性脑水肿的动物实验研究

Medication of Traumatic Brain Edema in Rats

为研究临床常用的甘露醇和速尿对创伤性脑水肿的治疗作用 ,因改良Feeney自由落体装置致大鼠创伤性脑水肿 ,分别用不同剂量的甘露醇、速尿及 2药合用进行治疗。通过对血 脑脊液屏障通透性的观察、脑组织含水量的测定及光镜、电镜下的病理组织学检查 ,判定各治疗组的疗效。结果 :大剂量甘露醇治疗组的脑水肿蓝染面积和脑组织含水量下降最明显 ,光镜及电镜下观察脑组织损伤最轻 ,但随着治疗次数的增加 ,其治疗效果反而下降 ;小剂量甘露醇和速尿合用组的脱水作用虽不如大剂量甘露醇组强 ,但随着治疗次数的增加 ,其治疗效果并未明显下降。结果提示 :甘露醇对于急性创伤性脑水肿的治疗效果最佳 ,使用甘露醇 5～ 6次后应使用小剂量甘露醇 +速尿治疗 ,单独应用速尿效果欠佳。

To study the different medication of traumatic brain edema in rats, 54 rats in which the models of traumatic brain edema were induced by Feeney's extradural impact were divided into six groups randomly. Different groups were treated with special drugs and different dosages. The study observed the changes of the blood brain barrier with Evans Blue and the water content of the brain after the brain injury in rats. Injury areas were observed by pathological techniques in the experiment. The decreases of injury area and the water content of the brain after the brain injury were the most evident in the groups that were treated with large dosage mannitol. It was proved by the pathological observation that brain injury was the lightest in the asme groups. Curative effect of little dosage mannitol and frusemin used together can last for a long time. Mannitol is the most effective drug in treating acute traumatic brain edema , but little dosage mannitol and frusemin used together is the best choice after mannitol was used solely five to six times.