山丹芍药汤化学成分的分离与鉴定(Ⅱ)

Isolation and Elucidation of Chemical Constituents of Shandanshaoyao Decoction (Ⅱ)

目的 :对山丹芍药汤化学成分进行研究。方法 :水提醇沉法制备样品 ,氯仿、乙酸乙酯和水饱和正丁醇提取 ,大孔吸附树脂、硅胶和聚酰胺柱分离 ,物理、化学和光谱学方法鉴定化合物。结果 :从水饱和正丁醇提取部分和水溶性部分分得 9个化合物 ,鉴定为牡荆素、槲皮素、金丝桃苷、芦丁、芍药苷、原儿茶醛、儿茶素、丹参素和柠檬酸。结论 :上述成分系首次从该复方中分离报道。

Objective:To study the chemical constituents of Shandanshaoyao Decoction.Method:The sample was prepared by boiling water, then precipitated by 75% of ethanol. The ethanol soluble fraction was extracted by chloroform, ethyl acetate and n-butanol, respectively. The extracts were isolated using macroreticular resin, silica gel and polyamide column chromatography, and then the chemical constituents were identified by chemical and spectroscopic methods.Result:Nine compounds were isolated from the n-butanol extract and water soluble fraction, and elucidated as vitexin, quercetin,hyperoside,rutin, paeoniflorin, protocatechaldehyde, (+)-catechin, danshensu and citric acid respectively.Conclusion:The chemical constituents of traditional Chinese medicinal preparations Shandanshaoyao Decoction were reported for the first time. [