人参、西洋参及三七参指纹图谱鉴别

HPLC Fingerprints Identification of Panax ginseng C. A. Mey., P. quinquefolin L. and P. notoginseng (Burk. F. H. Chen)

目的 :对人参、西洋参和三七参进行指纹图谱研究。方法 :PolarisC18 A ,乙腈 水梯度脱 ,流速 1.5ml·min-1,检测波长 2 0 3nm。结果和结论 :3种参的皂苷类成分均得到很好分离 ,该法可有效鉴别人参、西洋参和三七参。

Objective:The method was established to identify Panax ginseng, P. quinquefolium and P. notoginseng.Method:Polaris C 18 -A analytical column (250 mm×4.6 mm,5 μm);acetonitrile-water as gradient eluent, flow rate 1.5 ml·min -1 ,detective wavelength at 203 nm.Result and Conclusion:The fingerprints of P. ginseng, P. quinquefolium and P. notoginseng were obtained,and all ginsenosides were analyzed perfectly. The peak height ratio of ginsenoside Rg 1 and ginsenoside Re was a suitable character to differentiate the three species from each other. [