微菌落技术快速定量测定矿泉水中细菌总数的初步研究

The Detection of Total Bacterial Number in Mineral Water by Microcolony Technique

目的 初步建立检测矿泉水中细菌总数的微菌落技术。 方法 以抽滤方式将待测水样 1ml接种于混合纤维素膜上 ,然后贴在琼脂平板上 37℃培养 12h ,取下膜透明处理、沙黄染色后显微镜记数。 结果 与常规平板计数法比较 ,差别无显著性。同时可测定细菌浓度低于 10 3cfu/ml的水样。结论 该法具有经济、快速。

Objective To establish a microcolony method for detecting the total bacterial number in Mineral water.Methods Znoculating 1ml of the sample water onto the Cellulose acetate/nitrate mixed filter membrane by vacuum pump.then placing the membrane on the surface of agar medium.Taking out the membrane after 12 hour incubation at 37 ℃,making it transparent and stain with safranine,and observing with microscope.Results There are no significant differences between the microcolony technique and the plate counting method.Moreover,it has been improved for testing the sample with detection the bacterial numbers lower than 10 3 cfu/ml.Conclusions This method has the advantage of rapid,low-cost and easy results preserveing.