17β-雌二醇和晚期糖化终末产物对大鼠成骨细胞增殖和分化的影响

EFFECTS ON 17β- ESTRODIOL AND ADVANCED GLYCOSYLATION END PRODUCT ON OSTEOBLASTIC CELL PROLIFERATION AND DIFFERENTIATION

目的探讨不同浓度的 17β 雌二醇 (17β estrodiol)对成骨细胞增殖分化的影响及雌激素 (estrogen)与晚期糖化终末产物 (advancedglycosylationend product ,AGE)共存对成骨细胞的作用。 方法用不同浓度的 17β 雌二醇加入大鼠成骨细胞培养体系 ,不同作用时间 ,观察对大鼠成骨细胞增殖分化的影响 ,以及 17β 雌二醇与AGE共存时对成骨细胞增殖的影响。结果 17β 雌二醇 >10 -9mol/L主要表现为对大鼠成骨细胞增殖的抑制作用 ,10 -10和 10 -11mol/L则显著促进大鼠成骨细胞的增殖 (P <0 .0 1,P <0 .0 5 ) ;10 -12 ～ 10 -14 mol/L的 17β 雌二醇培养48h显著抑制成骨细胞增殖 (P <0 .0 1) ,10 -6～ 10 -8mol/L 17β 雌二醇存在下培养 2 4、48、72h ,均表现出显著抑制成骨细胞的分化 ;10 -10 mol/L 17β 雌二醇与 40 0～10 0 0mg/LAGE共同存在 ,培养 2 4h则均表现出促增殖作用(P <0 .0 1)。结论雌激素在生理浓度对大鼠成骨细胞增殖产生抑制作用 ,低于生理浓度具有促增殖活性 ,浓度进一步降低 ,再次出现抑制作用 ,且适量浓度的雌激素与高浓度AGE共存时 。

ObjectiveTo explore effects of 17β estrodiol at different concentrations on proliferation and differentiation of osteoblastic cells,and the effects of coexistence of estrogen and advanced glycosylation end product(AGE).MethodsOsteoblastic cells from rats were incubated by adding 17β estrodiol at different doses in different time ,or by adding simultaneously 17β estrodiol and AGE,and then cell proliferation and differentiation was observed.ResultsAfter incubation in different time, more than 10 -9 mol/L 17β estrodiol chiefly induced the inhibition of cell proliferation, while 10 -10 and 10 -11 mol/L 17β estrodiol significantly stimulated osteoblastic cell proliferation. After 48 h incubation, 10 -12 ～10 -14 mol/L 17β estrodiol induced the significant inhibition of cell proliferation.When alkaline phosphatase (ALP) activity was studied, cells exposed to 10 -6 ～10 -8 mol/L 17β estrodiol decreased their ALP activity after 24 h, 48 h and 72 h incubation respectively. After 24 h incubation, there was significant increase of cell proliferation in coexistence of 10 -10 mol/L 17β estrodiol and AGE whose doses ranged from 400 to 1 000 mg/L.ConclusionPhysiological level of 17β estrodiol (about 10 -9 mol/L) inhibited osteoblastic cell proliferation. Less than physiological contents of 17β estrodiol significantly stimulated cell proliferation. In the early incubation, coexistence of 10 -10 mol/L 17β estrodiol and higher contents of AGE remarkably stimulated cell proliferation. MeSH estrogen;advanced glycosylation end product;osteoblastic cell;cell proliferation; cell differentiation