摘要
目的 :研究 (-)黄皮酰胺对记忆障碍、神经可塑性、神经细胞凋亡和脑内蛋白磷酯酶活性的影响。方法 :采用Morris水迷宫、电生理、生化学等方法以及在三种凋亡模型上观察 (-)黄皮酰胺的上述作用。结果 :(-)黄皮酰胺能改善 β 淀粉样肽 (2 5 3 5 )引起的空间辨别障碍 ,同时提高脑皮层的胆碱乙酰转移酶 (ChAT)活性。在麻醉和自由活动大鼠 ,(-)黄皮酰胺能诱导LTP的形成和增加高频电刺激 (HFS)引起的LTP的幅度。给断乳小鼠和成年大鼠口服 (-)黄皮酰胺 2 8和 10d ,能使海马突触密度和苔藓神经纤维末梢发芽数增加。在三种凋亡模型上 (-)黄皮酰胺均显示明显的抗细胞凋亡作用。 (-)黄皮酰胺还能提高脑内蛋白磷酸酯酶Calcineurin和Calpain的活性。结论 :(-)黄皮酰胺有望开发成为抗老年痴呆新药。
Objective:To observe effects of (-) clausenamide on memory impairment, neural plasticity, apoptosis and protein phosphatase activity. Methods:Morris water maze, electric physiological and biochemical methods and three apoptotic models were used in the present studies. Results:(-) Clausenamide could improve Aβ (25 35) imduced impairment of spatial descrimination, incrase cortical ChAT activity and potentiat basic synaptic transmission and HFS induced LTP amptitude as well as increase hippocampal synapses and Mossy fiber sprouting. The protein phosphatas calcineurin and calpain activity in hippocampus and cortex were also increased by (-) clausenamide. Conclusion:(-) clausena mide is considered as a promissing antidementia drug.
出处
《医药导报》
CAS
2001年第7期403-404,共2页
Herald of Medicine
基金
国家自然科学基金委重大项目 (编号 :2 9790 1 2 0 )
关键词
黄皮酰胺
记忆障碍
苔藓神经末梢发芽
抗老年痴呆
蛋白磷酸酯酶
治疗
Clausenamide
Aβ-(25-35) induced memory impairment
Synapses
Mossy fiber sprouting
Antidementia
Protein Phosphatase
