新生儿缺氧缺血性脑病NO、GSH-PX和TNF的含量测定及其临床意义

Testing the concentrations of NO, GSH-PX and TNF of newborn infants with HIE

目的 探讨新生儿缺氧缺血性脑病 (HIE)血浆和脑脊液 (CSF)中的一氧化氮 (NO)和谷胱甘肽过氧化物酶(GSH -PX)和肿瘤坏死因子 (TNF)含量变化及其与HIE不同时期和不同程度间的相关关系。方法 对HIE患儿第 3天的CSF和出生初入院 (HIE 2h内 )、第 1天、第 2天、恢复期的血浆中NO、SOD进行检测 ,并与正常对照组比较 ;分析NO、GSH -PX、TNF的变化原因和意义。结果 HIE患儿血浆中第 1天的NO含量最高 ,而GSH -PX相反 ;初入院、第 1天、第 3天的血浆中NO、GSH -PX含量与正常对照组对比均有显著性差异 (P <0 0 1) ,而恢复期中NO、GSH -PX含量与正常对照组对比无显著性差异(P >0 0 5 ) ,血浆和HIE第 3天的CSF中NO和GSH -PX水平均呈负相关 ;病情越重NO浓度越高 ,GSH -PX越低。急性期血浆TNF显著高于对照组 (P <0 0 1) ,恢复期两组无显著性差异 (P >0 0 5 )。结论 NO、GSH -PX和TNF参与HIE的发病过程 ,在HIE的发病过程中起着重要作用 ;检测血浆和CSF中NO、GSH -PX。

Objection To study the expression of the nitric oxide(NO),Glutathion Peroxidase(GSH-PX)and Tumor necrosis factor(TNF)in plasma and cerebrospinal fluid(SCF)of neonate hypoxic-ischemic enceph alopathy(HIE)and the relations among their expressions in different periods and degrees of HIE.Methods Tested the concentrations of NO and GSH-PX and TNF in CSF of newborn infants suffered HIE 3 days and in plasma of newborn infants suffered HIE just attacked instant(HIE within 2 hours),1 day,3 days and restoring stage and Compared with them of normal contrast term.Analysed the reasons and significance of NO,GSH-PX and TNF changes Results The NO concentration is the highest in plasma of newborn infants suffered HIE 1 day.The GSH-PX concentration is the opposite .There are founded to have significant differences( P <0 01) after comparing the NO concentrations and the GSH-PX concentrations in plasmas of newborn infants suffered HIE just attacked instant ,1 day,3 days with of normal contrast team respectively.But there isn't significant difference( P >0 05)between them in plasmas of HIE on restoring stage and of normal contrast team.It is negative correlation whatever between the NO concentration and the GSH-PX concentration in plasma or in CSF of newborn infants suffered HIE 3 days.The more serious the disease is,the higher the NO concentration is,the lower the GSH-PX concentrations is. There is significant difference between the TNF concentrations in plasma of HIE and normal contrast team during acute periods,but there isn't difference in restoring period.Conclusion NO, GSH-PX and TNF play along with the course of HIE and play a important role in neonate HIE.Testing the concentration of NO, TNF and GSH-PX in plasma and in CSF can also help to judge the degree of disease and the course of disease.