反相高效液相色谱法测定人体内氟比洛芬含量

Determination of flurbiprofen in human plasma by RP-HPLC

目的 :建立人血浆中氟比洛芬反相高效液相色谱测定法。方法 :采用外标法 ,样品处理采用溶剂萃取法 ,色谱柱 :Ir regular HC18柱 ,流动相 :甲醇 7.4 2mmol·L-1磷酸液 ( 75∶2 5 ) ,流速 :1.0ml·min-1,检测波长 :2 4 7nm ,灵敏度 :0 .0 1AUFS ,进样量 :2 0 μl。结果 :本法线性范围 0 .0 5～ 2 0 .0 μg·ml-1,最低检测限 0 .2ng ,绝对回收率91.0 1% ,相对回收率 98.3 1% ,日内和日间差分别为 2 .93 %和 5 .73 %。结论 :本测定方法简便易行 ,符合分析测定要求。

OBJECTIVE To establish an RP HPLC method to measure flurbiprofen in plasma.METHODS The sample was pre treated with solvent extraction. Flurbiprofen was assayed by HPLC with the following.Irregular H C 18 column, UV detective wavelength 247 nm,flow rate 1.0 ml·min -1 , and the mobile phase of methanol - 7.42 mmol·L -1 phosphoric acid aqueous solution (75∶25).RESULTS Linear range was 0.05 ～ 20.0 μg·ml -1 ,detection limit was 0.2 ng,recovery was 98.3 %.CONCLUSIONS The method is convenient and practicable.