反相高效液相色谱法同时测定祛痹丹胶囊中士的宁和马钱子碱的含量

Determination of strychnine and brucine in QuBiDan capsule by RP-HPLC

目的 :建立祛痹丹胶囊中士的宁和马钱子碱的反相高效液相分析法。方法 :色谱柱C18( 10 μm ,4 .6mm× 2 5 0mm) ;流动相为 0 .0 1mol·LKH2 PO4∶甲醇 乙腈 ( 73∶2 7∶4 0 ) ,流速 1ml·min-1;检测波长 2 5 4nm。结果 :士的宁和马钱子碱在 0 .0 2～0 .2mg范围内呈良好线性关系。士的宁r =0 .9999,平均回收率 10 0 .2 6% ,RSD为 1.15 % ;马钱子碱r =0 .9995 ,平均回收率99 .95 % ,RSD为 1.2 5 %。结论 :该分析法为一种灵敏、准确而简便的分析方法。

OBJECTIVE To establish an HPLC method for assay of strychnine and brucine in QuBiDan capsule.METHODS With C 18 column (10 μm, 4.6 mm×250 mm), 0.01 mol·L -1 KH 2PO 4 MeOH CH 3CN(73∶27∶40)as mobile phase,the flow rate of which is 1 ml·min -1 ,detection at 254 nm.RESULTS Linear range of strychnine and brucine was 0.02 ～ 0.2 mg.The recovery rate of strychnine and brucine was 100.26 %( RSD = 1.15 %) and 99.95 % ( RSD = 1.25 %) respectively.CONCLUSIONS This method provides a simple and feasible way to control the quality of QuBiDan capsule.