纳米级磁性微粒的制备及固定化纤维素酶的研究

Studies of Preparation and Characterization of Magnetic Gelatin Microspheres and Immobilization on Cellulase

研究了明胶改性新途径 ,制备了核壳式纳米级磁性明胶微粒。以此为载体对纤维素酶进行固定化 ,确定了固定化工艺条件 ,研究了磁性固定化酶的最适温度、最适pH ,使磁性固定化酶的热稳定性。

The magnetic gelatin composite microspheres have been prepared.The microspheres, containing magnetite cores surrounded by chemisorbed gelatin, have 7～100 nm diameter.The conditions of cellulase immobilized on the magnetic gelatin composite microsphere were studied.The results showed that the activity of MIE was 489 U/g carrier.The activity recovery was 76 7%.The optimum temperature,optimum pH values, stabilities to heat and operational stability were all studied and compared with the native enzyme