恶性胸腹水细胞FCM-DNA定量分析在临床诊断中的应用

Diagnostic evaluation of the FCM-DNA quantitative analysis about the cell of malignant hydrothorax and ascites

目的 :用流式细胞仪对胸腹腔积液患者同一次胸腹水细胞进行DNA含量测定 ,并与胸腹水脱落细胞学检查进行对比分析。方法 :取 1 0 6 /ml细胞悬液 ,离心弃上清 ,加入荧光染料碘化丙啶液 ,用美国B -D公司的FACSCalibur型流式细胞仪进行测定 ,用FMu -5型微型玻片离心沉淀法脱落细胞学检查。结果 :流式细胞仪检测 40例恶性胸腹水细胞DNA非二倍体出现率为 90 % (36 / 40 ) ,脱落细胞学的检出率为 87 5%(35/ 40 )。结论 :两种方法联合应用检出率为 92 5% (37/ 40 ) ,显著提高良恶性胸腹水的判断率。

Objective:The same sample that the cell of hydrothorax and ascites was tested for DNA content by Flow cytometry (FCM),the results were compared with the method of exfoliative cytology.Method:To take 10 6/ml cell suspension,it be centrifugatized and was the supernatant fluid,abandoned the test was performed after we put propidium iodide into it by FACS calibur FCM (made in USA),while exfoliative cytology test was made.Results:The detection rate of DNA non-diploid is 90% by FCM and 87.5% by the method of exfoliative cytology.Conclution:The DNA nondiploid detection rate is 92.5% by use FCM and method of exfoliative extology for compensation.So can more correctivey identify the benign or malignant hydrothorax and ascites.