胃镜消毒模拟试验──清洗与消毒时间探讨

The Imitative experiment on gastroscopic disinfection── determination of cleaning and disinfectant time

目的探讨胃镜清洗与消毒浸泡的有效时间。方法采用 1μ g/ml乙型肝炎表面抗原 (HBsAg)和浓度为 3× 108/ml的金黄色葡萄球菌、大肠杆菌、绿脓杆菌悬液均匀地涂布在胃镜的镜身、活检孔道、活检钳，对胃镜按常规进行清洗 3min后分别在消毒 3min、 5min、 10min，收集清洗前、清洗后及消毒后不同时间段的标本，测其 HBsAg和致病菌。结果清洗前能检测出致病菌和 HBsAg,彻底清洗后测不出 HBsAg但能检测到致病菌，用适滴 (碱性活性戊二醛 )浸泡 3min后能杀灭致病菌及 HBsAg。结论彻底清洗与在 2％戊二醛液中浸泡 3min能有效杀灭致病菌与 HBsAg。

Objective To determine the cleaning and the shortest disinfectant time for gastroscope Methods 1μ g/ml HBsAg and 3× 108/ml bacteria of Aureus Staphylococci(ATCC 6538),Escherichia coli(ATTC 8099),Bacterium earuginosum(ATCC 27853)were artificially spreaded on the body of gastroscope ,biopsy hole and biopsy clamp.The gastroscope was routinely cleaned and disinfected.HBsAg and pathogens in the samples,which collected before and after cleaning the gastroscope and 3min,5min,10min after disinfection,were tested by using ELISA and bacteria culture,respectively.Results Pathogen and HBsAg can be detected before cleaning the gastroscope.Pathogen can also be detected,but HBsAg disappears after cleaning.Both HBsAg and pathogen was negative 3 min after gastroscope was immersed in glutaraldehyde.Conclusion The tests for pathogens and HBsAg can be negative after gastroscope was cleaned and immersed in 2％ glutaraldehyde for 3 min.