在体监测精神分裂症模型大鼠纹状体多巴胺的释放量及氟哌啶醇对其作用

DOPAMINE RELEASE IN Cpu IN SCHIZOPHRENIC RATS IN VIVO AND EFFECT OF HALOPERIDOL

①目的 在体监测精神分裂症模型大鼠纹状体多巴胺 (DA)的动态变化 ,以探讨精神分裂症的发病机制及氟哌啶醇的作用。②方法 经腹腔给大鼠注射苯环己哌啶 (PCP)制作精神分裂症动物模型 ,用快速周期伏安法 (FCV)在体测量模型大鼠纹状体DA的释放量。③结果 PCP模型大鼠纹状体DA的释放量左侧为 (3.95±0 .6 3) μmol/L ,右侧 (4 .0 3± 0 .5 8) μmol/L ,明显高于对照组的 (1.32± 0 .2 7) μmol/L和 (1.2 6± 0 .2 9) μmol/L(t =2 2 .5 9,12 .19,P <0 .0 0 1)。短期给予氟哌啶醇治疗后 ,纹状体DA的释放量左侧为 (2 .6 5± 0 .5 6 ) μmol/L ,右侧(2 .5 3± 0 .32 ) μmol/L ,较PCP组 (4 .33± 0 .44 ) μmol/L和 (4 .45± 0 .5 6 ) μmol/L无明显改变 (F =18.5 5 ,18.34 ,q =2 .85 ,2 .39,P >0 .0 5 )。而长期给予氟哌啶醇后纹状体DA的释放量左侧为 (2 .36± 0 .5 7) μmol/L ,右侧为 (2 .2 9±0 .75 ) μmol/L ,较PCP组的 (4 .31± 0 .6 0 ) μmol/L和 (4 .5 8± 0 .6 4) μmol/L明显降低 (F =6 9.0 4,5 9.80 ,q =4.79,5 .43,P <0 .0 0 1)。④结论 PCP精神分裂症模型大鼠纹状体DA功能亢进 。

Objective To study the etiology of schizophrenia and effects of haloperidol , we monitored the dynamic changes of dopamine(DA) release in rat caudate putmen(Cpu). Methods We used fast cyclic voltammetry(FCV) technique to detect the release of DA in Cpu of phencyclidine(PCP) induced schizophrenic rats. Results DA release in Cpu was apparently higher in PCP induced model rats,(3.95±0.63)μmol/L for the left side and (4.03±0.58)μmol/L for the right side,than that in the control,(1.32±0.27)μmol/L and(1.26±0.29)μmol/L(t=22.59,12.19,P<0.001). After a short term haloperidol treament, DA release in Cpu was not changed; after a long term haloperidol treament , DA release in Cpu in PCP induced model rats reduced,(2.36±0.57)μmol/L and(2.29±0.75)μmol/L,compared with those without therapy,(4.31±0.60)μmol/L and(4.58±0.64)μmol/L(F=69.04, 59.80,q=4.79, 5.43,P<0.001). Conclusion DA system is hyperactive in Cpu in schizophrenic rats, and haloperidol could effectively reduce the release of DA.