3种反义核酸增加白血病细胞K562对顺铂的敏感性

Three kinds of antisense oligodeoxynucleotide enhance the sensitivity of leukemia cell K562 to cisplatin

目的 :研究和探索hTERT、bcl- 2、c -myc基因的反义核酸对K5 6 2细胞顺铂 (cisplatin)敏感性影响 ,以期增强顺铂对白血病疗效。方法 :采用MTT法检测 3种反义核酸和顺铂对K5 6 2细胞抑制率。结果 :顺铂 2 0 μmol/L对K5 6 2细胞抑制率为 17 17%± 1 36 % ,顺铂 +hTERT反义序列对K5 6 2细胞抑制率为 2 5 41%± 1 77% ,顺铂 +bcl- 2反义序列对K5 6 2细胞抑制率为 2 6 18%± 1 43% ,顺铂 +c -myc反义序列对K5 6 2细胞抑制率为 2 8 2 9%±1 0 5 %。结论 :hTERT、bcl- 2、c -myc基因的反义核酸能显著提高K5 6

AIM: To investigate whether antisense oligodeoxynucleotides of hTERT?bcl-2 and c-myc could enhance the sensitivity of leukemia cell K562 to cisplatin. METHODS: The inhibiting effects of cisplatin and cisplatin plus antisense oligodeoxynucleotide on K562 cells were determined by MTT. RESULTS: The inhibiting rate of 20 μmol/L cisplatin to K562 cell is 17.17%±1.36% and it becomes 25.41%±1.77% ,26.18%±1.43% and 28.29%±1、05%, respectively, as combinated with antisense oligodeoxynucleotide of hTERT?bcl-2 or c-myc. CONCLUSION: These results indicated that antisense oligodeoxynucleotides of hTERT?bcl-2 and c-myc enhanced efficacy of cisplatin in K562 leukemic cells.