黄连提取工艺的研究

Study on Extraction Process of Rhizoma Coptidis

目的 :通过绘制黄连在不同溶剂中的总碱浸出 时间曲线 ,确定其正交优选试验中的因素及水平。方法 :以黄连总碱浸出量为指标 ,绘制总碱浸出 时间曲线 ;以总碱浸出量、盐酸小檗碱浸出量、浸膏得率为评价指标 ,用正交试验法优选黄连提取工艺。结果 :表明黄连总碱的浸出量与溶剂量、溶剂种类、浸提时间等因素有关 ;黄连最佳提取工艺 :5 0 %乙醇回流提取 3次 ,每次 10倍量溶剂 ,提取 2h。结论 :总碱浸出一时间关系的考察对正交试验设计具有指导意义 ;

Objective: To ascertain the factors and levels in the orthogonal design by drawing the extraction amount time curve of total alkaloid of Rhizoma Coptidis in different solvents. Methods:The extraction amount time curve of total alkaloid was plotted with the extraction amount of total alkaloid as a marker. The orthogonal design was used to select the optimum extraction process according to the contents of total alkaloid and berberine, and extract yield. Results:The extraction amound of total alkaloid related to volume and kinds of solvent and extraction time. The optimum exttraction process was as follows: adding 50% alcohol into meterials and refluxing and extracting for 3 times, 120 min every time. Conclusion:The investigation on the relation between total alkaloid extraction amount and time is of an instructive significance in orthogonal design. The optimum extraction process of Rhizoma Coptidis will provide an experimental basis for industrial production.