流式细胞仪法评价锶磷灰石细胞毒性

To evaluate the cytotoxicity of strontium substituted hydroxyapatite by flow cytometry

目的 尝试用流式细胞仪法探讨锶磷灰石的细胞毒性。方法 用流式细胞仪测定锶磷灰石浸提液作用细胞后细胞群落的DNA增殖指数。结果 增殖指数随着材料掺锶量的增加而增大 ,其中含锶10 0 %的纯锶磷灰石增殖指数最大、1%锶磷灰石的增殖指数最小。结论 随掺锶量增加后锶磷灰石的细胞毒性也略增大。

Objective To evaluate the cytotoxicity of strontium substituted hydroxyapatite by flow cytotetry.Methods Assess the cell DNA Proliferation Index(PI) of cell groups effected by strontium substituted hydroxyapatite with variety amounts of strontium through flow cytometry. Results The results showed that the PI was increased with the adding of Strontium. The PI was highest when the Strontium concentration was 100%,and smallest when the concentration was 1%. Conclusion The cytotoxicity of Strontium substituted hydroxyapatite was slightly increased with the adding of Strontium.