血管紧张素原基因M235T多态性与老年脑梗死的相关性研究

Association of M235T polymorphism of the angiotensinogen gene with cerebral infarction in old patients

目的 探讨血管紧张素原 (angiotensinogen ,AGT)基因M2 35T分子变异与脑梗死 (cerebralinfarction ,CI)之间的关系。方法 采用聚合酶链反应 (PCR)及限制性片段长度多态性分析 (RFLP)法对 75例CI、48例健康对照进行了AGT基因M2 35T多态性检测。结果 CI组AGT基因T2 35等位基因频率为 0 .78,2 35TT基因型频率为 0 .6 4,与对照组(分别为 0 .6 0 4和 0 .375 )比较差异具有显著性 (χ2 =8.82 ,P=0 .0 0 3;χ2 =8.2 7,P =0 .0 0 4)。校正了CI的几种危险因素 (血总胆固醇、血糖及年龄 )后 ,2 35TT基因型仍可使CI发生的危险性增加 (分别为OR =3.2 89,P =0 .0 36 ;OR =2 .49,P =0 .0 2 3)。结论 AGT基因 2 35TT型可能是脑梗死发病的独立危险因素。

Objective To investigate the association between angiotensinogen (AGT) gene M235T polymorphism and cerebral infarction(CI).Methods AGT gene M235T polymorphism was examined in 75 cases of CI and 48 healthy controls by polymerase chain reaction (PCR) amplification and restriction fragment length polymorphism(RFLP).Results The frequencies of AGT gene T235 allele and 235TT genotype were 0.78 and 0.64 respectively in CI group.Compared with those of the control group ( 0.604 and 0.375 respectively),the difference was statistically significant(χ 2=8.82,P=0.003;χ 2=8.27,P=0.004).After three CI risk factors (blood sugar,blood total cholesterol and age) were adjusted,235TT genotype could still raise the incidence of CI (OR=3.289,P=0.036;OR=2.49,P=0.023).Conclusion 235TT genotype of the AGT gene might be an independent risk factor of CI.