摘要
目的 了解肺癌患者明胶酶A基因表达的情况。方法 19例肺癌患者术中取癌组织及正常肺组织 ;提取组织总RNA ;用共扩增逆转录定量PCR方法测定其基因表达水平。结果 肺癌组织明胶酶A(MMP2 )的基因表达明显高于正常肺组织 ;肺癌组织MMP2 基因表达水平与肿瘤大小 ,肿瘤组织类型无关。结论 明胶酶A在肺癌的浸润中可能起重要作用。
Objective To investigate gelatinase A(MMP_2) gene expression in patients with pulmonary carcinoma.Methods Cancerous tissue and normal lung tissue were collected from 19 patients with lung cancer and total RNA was isolated from tissues.Gelatinase A gene expression level was measured by quantitative analysis of reverse transcription polymerase chain reaction.Results Gelatinase A gene expression level in cancerous tissue was much higher than that of normal tissue.MMP_2 gene expression level had no correlation with tumor size and histological type of lung cancer.Conclusion Gelatinase A might play an important role in invasion of pulmonary carcinoma.
出处
《中国肿瘤临床与康复》
2001年第4期21-22,共2页
Chinese Journal of Clinical Oncology and Rehabilitation
关键词
肺癌
明胶酶A
基因表达
gelatinase A
gene expression
pulmonary carcinoma
