重庆地区汉族人肿瘤坏死因子-α基因的多态性与急性肺损伤

Polymorphism of tumor necrosis factor alpha and its relation with acute lung injury in people of Han nationality in Chongqing area

目的 :探讨重庆地区汉族人肿瘤坏死因子α(TNFα)基因 30 8位点的多态性与急性肺损伤 (AL I)的相关性。方法 :对 5 9例 AL I患者用放射免疫分析法 (RIA)测定 TNFα含量 ,用等位特异性聚合酶链反应(AS PCR)方法分析血清 TNFα基因多态性变化 ,并与 6 0名健康献血者对照。结果 :1AL I组 TNFα含量〔(1.883± 0 .10 6 ) μg/ L〕显著高于对照组〔(1.12 9± 0 .0 77) μg/ L〕,t=30 .16 ,P<0 .0 1;2 AL I组 TNFα(30 8A)等位基因频率 (33.9% )显著高于对照组 (6 .7% ) ,P<0 .0 1;3TNFα等位基因频率增高与 AL I的易感性相关联 ,比值比 (OR)为 7.36 8,95 %可信限 (CI)为 0 .370～ 8.6 2 0。结论 :重庆地区汉族人 TNFα基因30 8位点的多态性与 AL

Objective:To study the relationship between the308 locus of tumor necrosis factor alpha( TNFα ) genetic polymorphism and acute lung injury (ALI) in people of Han nationality in Chongqing area.Methods:Radioimmunoassay (RIA) was used to measure the content of TNFα in blood,and the allele specific polymerase chain reaction (ASPCR) was used to determine the308 locus alleles of TNFα genetic polymorphism in 59 patients with ALI and 60 healthy controls.Results:①The content of TNFα in ALI group (1 883±0 106) μg/L was significantly higher than that of control group (1 129±0 077) μg/L ( t =30 16, P <0 01).②There was a significant difference of the TNFα(308A) frequency in ALI (33 9%) and control group (6 7%), P <0 01.③The TNFα allele frequency was associated with ALI susceptibility (OR=7 368,95% CI=0 3708 620).Conclusions:These results suggest that TNFα308 locus genetic polymorphism is correlated with ALI susceptibility in people of Han nationality of Chongqing area.