氨基胍对内毒素诱发的兔急性肺损伤血清丙二醛的影响

The effects of aminoguanidine on serum malondialdehyde in rabbits with endotoxin-induced acute lung injury

目的 :观察氨基胍 (AG)对内毒素 (ET)诱发的兔急性肺损伤 (AL I)血清丙二醛 (MDA)水平的影响及其与血清一氧化氮 (NO)代谢产物 NO- 2 / NO- 3的关系。方法 :2 4只健康成年兔被均分为生理盐水组、ET组、AG组和 ET+AG组。 ET+AG组先静注 ET复制 AL I模型 ,再静滴 2 5 mg/ kg AG,共维持 3小时 ,观察不同时间点血清 NO- 2 / NO- 3及 MDA水平。实验结束后留取肺标本 ,观察病理损害情况。结果 :ET组 NO- 2 / NO- 3及MDA明显上升 ,持续静滴 AG后 NO- 2 / NO- 3及 MDA明显降低 ;组织病理观察表明 ET+AG组兔肺内炎症细胞渗出较少 ,肺水肿较 ET组减轻。结论 :AG可降低血清 NO- 2 / NO- 3及 MDA水平 ,减轻内毒素诱发的兔 AL

Objective:To observe the effects of aminoguanidine (AG) on serum malondialdehyde (MDA) in rabbits with endotoxininduced acute lung injury (ALI) and to observe the relationship between MDA and nitric oxide(NO) metabolism productions NO - 2/NO - 3 Methods:Twentyfour rabbits were divided into 4 groups ,they were saline group,endotoxin group,AG group and AG plus endotoxin group.In AG plus endotoxin group,endotoxin was injected to animals to make an ALI model firstly,then, 25 mg/kg AG was injected to sustain 3 hours.Serum MDA and NO - 2/NO - 3 were detected at different time points.By the end of the experiment,pathologic samples were obtained routinely.Results:After endotoxin injection,serum MDA and NO - 2/NO - 3 were increased significantly.The injection of AG could markedly decrease the amount of MDA and NO - 2/NO - 3 .Pathologic study showed that there were fewer inflammatory cells and less lung edema in AG plus endotoxin group than those in endotoxin group.Conclusions:AG could decrease MDA and NO - 2/NO - 3 in serum and had protective effects in rabbits with ALI induced by endotoxin.