血清HBV-DNA检测在招收飞行学员筛选中的意义

Significance of detection serum HBV-DNA in HBsAg negative cadet candidates

目的 调查在 EL ISA方法检测血清 HBs Ag阴性飞行学员中 ,血清 HBV- DNA的检出情况。 方法 用 EL ISA法检测 2 2 2 4名应招飞行学员血清 HBs Ag。其中阴性者进一步检测抗 - HBs、抗 - HBc、HBe Ag、抗 - HBe,并用聚合酶链反应 (PCR)检测 HBs Ag阴性者 HBV- DNA。 结果 在 2 2 2 4名应招飞行学员中 HBs Ag阳性 15 8例 ,阳性率 7.1% ;HBs Ag阴性者中 ,除单项抗 - HBs阳性血清模式外 ,其他各模式均有不同例数的人检测到 HBV- DNA。 结论 本研究表明 ,仅检测血清中 HBs Ag的表达状况 ,不足以将所有感染有 HBV者筛选出去 。

Objective To investigate the necessity of further screening serum HBsAg negative (ELISA method) cadet candidates by polymerase chain reaction (PCR) technique for detection of HBV DNA. Method In 2 224 cadet candidates serum HBsAg was examined by ELISA method, subsequently, anti HBs, anti HBc, HBeAg, anti HBe were examined, and HBV DNA detected in HBsAg seronegative candidates. Results In 2 066 seronegative candidates, HBV DNA positive was found in all subgroups except the positive for anti HBs only one. Conclusion This study indicated that ELISA method is not sensitive enough to exclude hepatitis B virus carriers, it should be replaced by PCR technique.