白细胞介素-2受体和糖皮质激素受体在脑神经元内共存

COEXISTENCE OF INTERLEUKIN-2 AND GLUCOCORTICOID RECEPTORS IN THE BRAIN NEURONS

目的 研究白细胞介素 2受体和糖皮质激素受体在大鼠脑内的分布及两者共存的可能性。 方法 免疫细胞化学双重标记技术 (PAP法与ABC法相结合 ) ,分别采用DAB和DBHC(二盐酸联苯胺 )呈色 ,前者为棕黄色 ,后者为深蓝色。 结果 白细胞介素 2受体和糖皮质激系受体广泛分布于大脑皮质、海马、下丘脑 ,脑干大部分运动性和感觉性核团。白细胞介素 2受体免疫反应产物位于细胞膜上 ;糖皮质激素受体免疫反应产物位于细胞核和胞浆内。脑内有较多的白细胞介素 2受体和糖皮质激素受体共存的神经元 ,它们在阳性神经元总数中所占的比例在不同的脑区有所不同。在大脑皮质约占 5 0 % ,在展神经核约占 30 %。 结论 免疫细胞因子和激素可通过各自的受体作用于同一个脑神经元而调节神经元的功能。本实验在受体水平为“免疫 神经 内分泌调节网络”

Objective To investigate the distribution of interleukin\|2 receptor (IL\|2R) and glucocorticoid receptor(GR) and identify coexistence of IL\|2R and GR in the rat brain. Methods The double labeling immunocytochemical technique(PAP method combined with ABC method), DAB and BDHC were used in the double labeling immunocytochemical method as the chromogens respectively. The reactive products of former was brown or yellow and later was black blue. Results IL\|2R and GR positive neurons were widely distributed in the cerebral cortex, hippocampus, thalamus, many motor and sense nuclei in the brain stem. The immunoreactive products of IL\|2R were found to be located on cell membrane and GR in nucleus and cytoplasm. There were a lot of positive double labeling neurons in the rat brain. The rate of double labeled cells in the total number of positive cells varied in different regions of brain, such as, 50 percent in cerebral cortex and 30 percent in nucleus of abducent nerve. Conclusion Immunogical cytokines and hormone could regulate the neuronal function through their corresponding receptors which coexisted in the same brain neurons. The present study might provide morphological evidence in the level of receptor for the immuno\|neuro\|endocrine network.