MMP-2和MMP-9在人脑原发性胶质瘤侵袭中的作用

THE FUNCTION OF MMP-2 AND MMP-9 IN PRIMARY HUMAN BRAIN GLIOMAS INVASION

目的 探讨基质金属蛋白酶MMP 2和MMP 9在原发性人脑胶质瘤侵袭行为中的作用。 方法 用免疫组织化学S P法检测MMP 2和MMP 9在胶质瘤和脑内转移瘤中的表达情况 ;同时通过测量CT影像上的瘤周水肿范围 ,并与免疫组织化学表达结果作对照比较。 结果 1 45例人脑胶质瘤标本中 ,MMP 2和MMP 9的瘤细胞阳性率分别为 35 5 %、6 2 2 % ,两者之间差别显著 (P <0 0 5 ) ;MMP 2、MMP 9在高度恶性胶质瘤标本中阳性率分别为 5 6 5 %、91 3% ,两者之间亦存在明显差别 (P <0 0 1) ;2 MMP 2的染色强度与胶质瘤侵袭指标———CT瘤周水肿范围无关 (P >0 0 5 ) ,而MMP 9的染色强度则与其有关 (P <0 0 5 ) ;但两者染色与否则均与其明显相关 (P <0 0 1) ;3 MMP 2、MMP 9两者在脑内转移瘤中表达率无明显差别 (P >0 0 5 ) ;也与高度恶性胶质瘤中的表达率无统计学差别 (P >0 0 5 )。 结论 MMP 2、MMP 9在人脑胶质瘤中可作为恶性表型及侵袭指标之一 ,亦可体现胶质瘤基质降解表型 ,但不能作为转移表型。其中 ,MMP

Objective To probe the function of matrix metalloproteinase 2(MMP 2) and matrix metalloproteinase 9(MMP 9) in primary human brain gliomas invasion. Methods The expression of MMP 2 and MMP 9 in glioma and brain metastases tissues was studied via the immunohistochemical stain.At the same time,the expression rate was compared with peritumoral low\|density range seen on CT\|scan. Results 1. In primary gliomas,the expression of MMP 2 and MMP 9 was positive in 35\^5%(16/45) and 62\^2%(28/45) of cases respectively.In high malignant glioma the positive rate (56\^5%) of MMP 2 is significantly lower than that (91\^3%) of MMP 9. 2. Peritumoral low\|density range seen on CT\|scan was correlated with the positive expression of MMP 2 and MMP 9 ( P<0\^01 ) and with the expression rate of MMP\|9( P <0\^01),but not with that of MMP\|2( P>0\^05). 3. In brain metastases the overexpression of MMP\|2 is not statistically different from that of MMP\|9( P>0\^05). The overexpression rate of both MMP\|2 and MMP\|9 in high malignant gliomas as compared with brain metastases is not statistically different( P>0\^05). Conclusion MMP\|2 and MMP\|9 may serve as indicators of invasion and malignant phenotype.Neither of them can represent the phenotype of metastasis,but both can represent that of matrix degradation.Moreover,MMP\|9 may play more important role in glioma invasion than does MMP\|2.