摘要
目的 研究K5 6 2细胞经氯化高铁血红素 (Hemin)诱导后在cDNA分子水平上的表达差异。 方法用限制性显示 (RD PCR)方法寻找K5 6 2细胞在经Hemin诱导后的cDNA差异表达片段。 结果 筛选到差异表达片段 4条 ,并进行了序列测定与同源性分析。 结论 K5 6 2细胞分化是多基因参与的结果 ,这些分化相关基因的共同作用使K5 6
Objective In the cDNA level to study the expressing difference of the K562 cells induced by Hemin. Methods Looking for the differential expressing cDNA fragments from the K562 cells induced by Hemin with the method of RD PCR(restriction display PCR). Results Four differential expressing fragments are screened, sequenced and analyzed of autoploidy. Conclusion The differentiation of K562 cells is involved with multiple genes and can be induced to normal under the control of these differentiatial related genes.
出处
《解剖学报》
CAS
CSCD
北大核心
2001年第4期357-361,共5页
Acta Anatomica Sinica
基金
国家自然科学基金资助项目 (3 9880 0 3 2 )
广州市重点科技攻关项目 (990 4480 2 2 )
