白介素-10基因疗法延长异体皮肤移植存活期及其机理初探

Preliminary study on effects and mechanism of IL-10 gene therapy on prolonging survival time of skin allografting in a murine model

目的 :探讨成纤维细胞介导的IL -1 0基因疗法对异体皮肤移植存活期的影响与可能的机理。方法 :小鼠腹腔内埋植高分泌IL -1 0的成纤维细胞 ,并移植异体鼠皮肤 ,观察排斥时间 ,测定受体鼠的淋巴细胞增殖反应、IFN -γ、IL -2、NK活性。结果 :成纤维细胞介导的IL -1 0基因疗法显著延长异体皮肤移植的存活期 ,并降低小鼠淋巴细胞的增殖反应 ,减少IFN -γ、IL -2的产生及降低NK活性。结论 :IL -1 0基因疗法可延长小鼠异体皮肤移植存活期 ,其机理与抑制机体的免疫效应因子有关。

Objective:To explore the effect and mechanism of fibroblast-mediated IL-10 gene therapy on prolonging survival time of skin allografting.Methods:Fibroblast(NIH3T3)carried highly produced IL-10 gene was implanted intraperitoneally in allografting skin murine model.The allograft skin survival time was observed,and immunologic indexes including the proliferation rate of spleen cells,IFN-γ,IL-2 and the activity of natural killer cells(NK)were determined in experimental murine model.Results:Fibroblasts-mediated IL-10 gene therapy could obviously prolong allografting skin survival time,and significantly inhibite proliferative reactivity of spleen cells to the lymphocyte mitogen concanavalin A,at the same time,it could decrease the production of IFN-,IL-2 and the activity of NK cells.Conclusion:Skin allograft survival time could be prolonged with the implantation of the fibroblastocyte transferred IL-10 gene,which is a result from IL-10 inhibition immune -reaction cytokine.