沙眼衣原体夹心ELISA检测方法的实验研究及临床应用

Experimental study and clinical application of sandwich ELISA for the detection of chlamydia trachomatis

目的 :建立沙眼衣原体 (CtSerotype)K -D抗原夹心ELISA实验室诊断方法 ,用于沙眼衣原体感染的诊断。方法 :选择高特异性和敏感性的沙眼衣原体McAb5D9和 1D6混合物为捕获抗体 ,以鸡胚卵黄囊吸收后的PcAD为标记抗体 ,进行沙眼衣原体夹心ELISA检测方法的实验研究。结果 :应用该方法对 1 92例疑为非淋菌性尿道炎的临床标本检测 ,其中 4 0例阳性 ,阳性检出率为 2 0 .81 % ;并以PCR作平行检测 ,4 2例阳性 ,阳性率为 2 1 .87% ,两种方法检测结果经统计学处理无显著差异 (P >0 .0 5)。结论 :夹心ELISA法检测沙眼衣原体 ,具有特异、敏感、快速、简便 ,不需要特殊仪器设备等优点 ,对沙眼衣原体感染的临床诊断和治疗具有重要的临床意义。

Objective:To establish sandwich ELISA for the detection of chlamydia trachomatis.Methods:The chlamydia trachomatis mixture of McAb5D9 and 1D6 was chosen as grasp antibody which was high specific and sensitive,PcAD was used as marked antibody and had been absorbed by chick embryo yolk sac.Results:192 samples suspected to be nongonococcal urethritis were detected by sandwich ELISA,40 were positive(20.81%).They were also detected by PCR to give a parallel comparision and 42 were found to be positive(21.87%).There was no significant diversity between the two methods.Conclusion:Sandwich ELISA is good in detection of chlamydia trachomatis.It's a specific and sensitive method with rapid results.It has great value in detection and treatment of chlamydeous infection.