部分肝切除对大鼠移植性肝癌生长的影响及其机制探讨

The Impact of Partial Hepatectomy on the Growth of HCC in the Murine Model

目的：研究部分肝切除（ＰＨ）对大鼠肝癌模型肿瘤生长的影响，并初步探讨其可能机制。方法：建立大鼠肝癌模型后，施行不同比例的部分肝切除，观察肿瘤生长变化，通过检测其细胞、体液免疫功能及免疫抑制因子变化，以初步探讨其可能机制。结果：部分肝切除后，接种的肿瘤随肝切除比例增加而生长明显加速，细胞免疫功能抑制程度随之加重，肝部分切除组血清ｓＩＬ－２Ｒ、ＴＧＦ－β、ＩＬ－１０在术后早期即与对照组具有显著差异。结论：ＰＨ能刺激接种肿瘤生长，可能与ＰＨ后免疫功能受抑、肝再生致细胞因子分泌异常等有关。

Objective: To study the impact of partial hepatectomy on the growth of hepatocellular carcinoma(HCC) in the murine model. Methods: Rats carrying the orthotopic HCC model were randomized to undergo 0%, 30% and 70% partial hepatectomy(PH). The changes of tumor size, cellular and humoral immune functions were evaluated and monitored. The levels of cytokines such as sIL-2R, TGF-β and IL-10 were detected by ELISA. Results: PH enhanced local tumor growth, associated with significant depression of the cellular immune function. The levels of sIL-2R, TGF-β and IL-10 increased in proportion to the extent of PH. Conclusions: The depression of immune function and the change in cytokine levels probably contributed to the accelerated tumor growth in the liver.