摘要
为了研究嵌合抗CD2 0基因工程抗体Fab′的抗肿瘤活性及其抗肿瘤机制 ,利用3H掺入法测定嵌合抗CD2 0Fab′对Raji细胞生长的影响 ,结果显示嵌合抗CD2 0Fab′对Raji细胞的生长具有抑制作用 ,利用流式细胞仪测定嵌合抗CD2 0Fab′诱导Raji细胞凋亡作用 ,结果显示嵌合抗CD2 0Fab′可诱导Raji细胞凋亡作用。这些实验结果证明嵌合抗CD2 0Fab′通过诱导Raji细胞凋亡的机制抑制Raji细胞生长。
The anti tumor efficacy of chimeric anti CD20 Fab′ fragment was analyzed by 3H UdR incorporation assay, result from 3H UdR incorporation assay showed that proliferation of Raji cells was inhibited by chimeric anti CD20 Fab′ fragment. Apoptosis induction of Raji cells by chimeric anti CD20 Fab′ fragment was analyzed by FACS, result from FACS analysis showed that chimeric anti CD20 Fab′ fragment was able to induce apoptosis of Raji.
出处
《高技术通讯》
EI
CAS
CSCD
2001年第7期20-22,共3页
Chinese High Technology Letters
基金
863计划资助项目 (863 10 2 0 9 0 3 0 3 )
