摘要
以玉米芯颗粒吸附桔青霉(Penicillium citrirum) 孢子,再用1 .5 % 的海藻酸钠包埋吸附固定化细胞玉米芯颗粒。于摇瓶中进行分批培养,实际结果表明:在固定化细胞产酶的条件下,培养基中淀粉水解糖浓度为9g/L,蛋白胨浓度为1g/L,摇瓶转速为180r/min ,产酶发酵周期为50h ,发酵液中核酸P1 的活力高达503U/ml。双载体固定化细胞经30 批次连续重复发酵产酶稳定在较高水平,固定化细胞粒子机械强度高。
Mycelia of Penicillium citrium were absorbed with corncob granules and wrapped up with 1.5% sodium alginate.The nuclease P 1 produced by the immobilized cells were cultured in shaking flasks.The shaking speed was 180r/min.The glucose and peptone contents in medium were 9g/L and 1g/L,respectly.After 50h culture,the nuclease P 1activity reached 510U/ml.In the repeated batch culture experiment,the immobilized cells maintained a high capacity and machinery strength after 30 batches.
出处
《中国粮油学报》
EI
CAS
CSCD
北大核心
1999年第5期44-46,共3页
Journal of the Chinese Cereals and Oils Association
基金
生物反应器工程国家重点实验室开放课题
关键词
桔青霉
固定化细胞
核酸酶P1
玉米芯
海藻酸钠
酶制剂
Penicillium citritum,immobilized cell,nuclease P 1,corncob granule,sodium alginate