双载体固定化桔青霉产生核酸酶P_1的研究

Nuclease P_1 Production by Immobilized Penicilium citrinum Cell

以玉米芯颗粒吸附桔青霉（Ｐｅｎｉｃｉｌｌｉｕｍ ｃｉｔｒｉｒｕｍ） 孢子，再用１ ．５ ％ 的海藻酸钠包埋吸附固定化细胞玉米芯颗粒。于摇瓶中进行分批培养，实际结果表明：在固定化细胞产酶的条件下，培养基中淀粉水解糖浓度为９ｇ／Ｌ，蛋白胨浓度为１ｇ／Ｌ，摇瓶转速为１８０ｒ／ｍｉｎ ，产酶发酵周期为５０ｈ ，发酵液中核酸Ｐ１ 的活力高达５０３Ｕ／ｍｌ。双载体固定化细胞经３０ 批次连续重复发酵产酶稳定在较高水平，固定化细胞粒子机械强度高。

Mycelia of Penicillium citrium were absorbed with corncob granules and wrapped up with 1.5% sodium alginate.The nuclease P 1 produced by the immobilized cells were cultured in shaking flasks.The shaking speed was 180r/min.The glucose and peptone contents in medium were 9g/L and 1g/L,respectly.After 50h culture,the nuclease P 1activity reached 510U/ml.In the repeated batch culture experiment,the immobilized cells maintained a high capacity and machinery strength after 30 batches.