单克隆免疫亲和柱-高效液相色谱法测定小麦中致呕毒素

Determination of Deoxynivalenol in Wheat by Affinity Cartridges-high Performance Liquid Chromatography

研究了单克隆免疫亲和柱 -高效液相色谱法测定小麦中致呕毒素的方法。样品通过水提取 ,提取液用免疫亲和柱净化 ,SpherisorbC18色谱柱分离 ,紫外检测器检测 ,外标法定量。对添加不同含量水平的致呕毒素 ,5次重复实验的平均回收率为 6 9.6 %～ 81.8%。变异系数 (CV)为 5 .7%～ 8.3%。检测低限为0 .0

A method for determination of deoxynivalenol (DON) in wheat by High Performance Liquid Chromatography (HPLC) is reported.The sample was estracted with water.Filtrate was cleaned up by DON test HPLC affinity cartridges.The deoxynivalenol was separated on Spherisorb C 18 column,detected by UV-deector and quantified by external standerd.Wheat samples were fortified with deoxynivalenol at four levels.Five replicate analyses were performed with each level.The recoveries of the deoxynivalenol were 69.6%～81.8%.The variation coefficients ranged over 5.7%～ 8.3% .The detection limit was 0.02mg/kg.