摘要
本文采用聚丙烯酰胺凝胶等电聚焦电泳(IEF),借助园盘电泳仪的简易装置,对正常 B 型G6PD 及变异型 G6PD 进行分析,获得了满意效果。
The existence of microheterogeneity of glucose-6-phosphate dehydrogenase(G6PD)in the human erythrocyt has already been reported(Der Kaloustian 1974).The results has been confirmed recently by isoelectric focusing in polyacrylamide gel.In this paper,we used the method which has been modified in some aspects to identify various G6PD variants,and came to interesting conclusions.We provide here our experimental data of two different G6PD types,GdB and Gd(-)Zhuang-Funing,Focusing of the enzyme gives additional in- formation concerning an accurate distinction among the genetic variants.
出处
《昆明医学院学报》
1991年第2期20-22,共3页
Journal of Kunming Medical College
关键词
等电聚焦电泳
红细胞
G6PD
变异型
Isoelectrofocusing electrophoresis
Isoelectric point
G6PD variant