激光梯度场中荧光关联谱学的实验研究

AN EXPERIMENTAL STUDY ON THE FLUORESCENCE CORRELATIONSPECTROSCOPY IN LASER GRADIENT FIELD

研究了荧光关联谱 (fluorescencecorrelationspectroscopy ,简称FCS)在FCS测量中外加激光梯度场的影响 .实验显示 ,微区内粒子的扩散时间随梯度场强度增加而增加 ,近似呈线性关系 .同时 ,微区内平均粒子数目随梯度场相应地增加 .粒子的极化率越大 ,相同条件下受到激光梯度场的影响就越大 .

This work studies experimentally the influence of a laser gradient field on fluorescence correlation spectroscopy measurements. Results show that the derived diffusion time for particles across the detection volume increases almost linearly with the strength of the, gradient field. The derived number of fluorescing particles in the detection volume also increases with the field. The larger the polarizability of the particles, the bigger the effects of the gradient field.