绞股蓝总皂甙对沙土鼠短暂性脑缺血后海马DND的影响

Effect of GPM on Hippocampal DND Following Transient Brain Ischemia in Gerbils

本实验通过结扎沙土鼠双侧颈总动脉10分钟再灌流7天,造成海马迟发性神经元死亡(Delayed Neuronal Death,DND)模型,用海马CA_1区神经元密度作为指标,观察绞股蓝总皂甙(GPM)对海马DND的影响。NS组、GPM-I组和GPM-Ⅱ组的CA_1区神经元密度分别是107.50±6.63、146.18±19.75和165.30±9.24。GPM-I组、GPM—Ⅱ组与NS组比较P<0.01,P<0.0001。均有高度显著性差异。结果表明绞股蓝总皂甙对短暂性脑缺血后海马DND有明显保护作用。

Hippocampal delayed neuronal death(DND)model was produced by bilateral carotid arteries occluded for 10 min with 7 day reperfusion.Effect of GPM on DND of gerbil hippocampus was examined by counting the neuronal density and histologic changes in CA_1sector.The average neuronal density((?)±SD)in NS group.GPM-I,GPM-Ⅱ group was 107.50±6.63/mu,146.18±19.75/mm and 165.30±9.24/mm respectively.Compared with NS contral group,both neuronal density in GPM-I group and GPM-Ⅱ group were significantly increased(P<0.01;P<0.0001;respectively).The results sugest that GPM significantly protected against hippocampal DND following transient cerebral ischemia in the gerbil.