鸭病毒性肝炎(DHV)诊断方法的研究

Studies on the Development of Diagnostic Method for the Deteetion of Duck Viral Hepatitis

鸭病毒性肝炎（ｄｕｃｋ ｈｅｐａｔｉｔｉｓ ｖｉｒｕｓ ＤＨＶ）病毒（ＡＴＣＣ株）用鸡胚胚传一代后，测出其鸡胚半数致死量（ＥＩＤ５０）为１０－５．５。７株ＤＨＶ毒株用鸡胚中和试验的结果是：胚传一代尿囊液死亡率为２／８－５／８，１：１０血清中和保护率为对 ８／８； １： １０００肝病料死亡率为 ３／８－ ８／８， １： １０血清中和保护率 ８／８，正常对照全部存活。雏鸭中和实验的结果是：２４小时内１：１０肝病料死亡率为３／８－８／８，１：５血清中和保护率为８／８，正常对照全部存活。

A duck hepatitis virus(DHV)was Propagated in chicken embryo,the embryo, the metal dose(LD50)in chicken embryo of DHV 10-5.5.The results of chicken embryo neutralization test of DHV were: The lethal rates of chorioallantoic fluid reanged between 2/8~ 8/8,the protection rates of ten fold diluted sera were 8/8;the lethal rates of infected liver supernatant diluted in 1: 1000 were 3/ 8~ 8/ 8 the serum protection rates of ten fold dilution were 8/ 8.and the control were all normal. The result of ducking neutralization test were: the lethal rates of infected liver supematant which were diluted 1: 1000 ranged between 3/ 8~ 8/ 8, the serum protection rates of five fold dilution were 8/8;and the control were all normal. A Preliminary diagnostic method was developed for the detection of duck hepatitis based on repeated tests.