从流行性出血热患者腹膜渗出细胞分离汉坦病毒

ISOLATION OF A HANTAVIRUS FROM PERITONEAL EXUDATE CELLS OF A PATIENT WITH HEMORRHAGIC FEVER WITH RENAL SYNDROME

本文报道从一例危重型EHF患者第10病日的腹膜渗出细胞分离出一株病毒,定名为GH716,用抗姬鼠型和抗家鼠型汉坦病毒(HTNV)单克隆抗体(McAb),对其抗原型别进行了鉴定,并与HTNV76-118株和R22株做了比较。同时检测了患者的血清抗体以及血淋巴细胞和尿细胞中的HTNV抗原。

A new strain of Hantavirus (HTNV) ,GH716, was isolated from peritoneal exudate cells (PEC) of a patient with severe form of hemorrha-gic fever with renal syndrome (HFRS) who lived in a highly endemic area in Guangan County, Sichuan, China. The isolate was propagated in Vero E6 cells. At each passage virus-infected cells were examined for HTNV antigen by the immunofluorescence (IF) technique with monoclonal antibodies ( McAb ) 25-1 and 84-1 against HTNV. From passage 5 on, fluorescent intensity tended to stabilize at 3+ and infectious titer reached to 105 TCID50/ml. Using McAb to 76-118 and Bl viruses, we found that strain GH716 was antigenically similar to strain 76-118 and different from strain R22 which was isolated from Rattus norvegicus, suggesting that strain GH716 may fall into the Apodemus type of HTNV. This is the first isolation of an HTNV from human PEC collected on the tenth day after onset of illness. The successful isolation of the GH716 strain seems to provide an alternative source of obtaining HTNV during the later stages of HFRS.