摘要
应用玻璃针或微吸管对分裂的金鱼(Carassius auratus)卵子进行不同分裂面的分割,或进行不同方向卵质的切割,或吸去部分卵黄质。结果是:采用上述三种方法后所发育的小鱼,都能得到一定数量的单尾鳍鱼。更有趣的是,一个已分裂的卵子分成两部分所形成的两条小鱼,其中一条形成单尾鳍,而另一条是双尾鳍。实验表明:金鱼单或双尾鳍的形成是与卵质有着密切关系的。这是因为,卵质中的某种物质——“调节因子”,存在于植物性半球卵黄囊中的一侧。该调节因子负有调控双尾鳍形成的作用:如果这种物质被分在分离的两部分卵子中,便形成两条双尾鱼;倘若进入其中之一,则会产生一条双尾,一条单尾。
The present paper deals with the formation of single caudal fin in the goldfish (Caras-sius auratus) by cutting the cleaving embryo, with a glass needle, into two parts along the desired plane or removing some cytoplasm from the developing embryo with a micropipettc. The experiment was carried out in following three groups.1. The fertilized egg was divided into two equal halves along the 1st or 2nd cleavageplane. The larval fish grown out of the fertilized eggs falls into three categories-(two)fish with double caudal fin, two with single caudal fin and the third group: one with double fin, one with single fin.2. Cutting off some cytoplasm along the plane from the base of blastomeres to the vegetal pole at different stages of development. It was founded that the embryos so operated gave rise to more larval fish with single caudal fin than those in the control group.3. Sucking out some yolk material using a micropipette. The experimental result showed that removing some yolk material induced the transformation of the caudal fin from the double to the single in the goldfish.The available data support the hypothesis that, in the goldfish egg cytoplasm, there is a kind of substance regulating the double caudal fin formation within the vegetal yolk sac, preferentially on one side of the egg. If both of the two half embryos divided at early cleavage stage possess the substance, they would both develop into two fish with double caudal fin. If only one of them contains it, there would be only one fish having double caudal fin. The counterpart cut from the same egg with no such substance, formed single caudal fin.
出处
《海洋与湖沼》
CAS
CSCD
北大核心
1989年第5期453-459,共7页
Oceanologia Et Limnologia Sinica
