syndecan-1蛋白的表达与肝细胞肝癌恶性表型的研究

CORRELATION BETWEEN SYNDECAN-1 PROTEIN EXPRESSION AND MALIGNANT PHENOTYPES OF HEPATOCELLULAR CARCINOMA

目的 探讨syndecan 1蛋白在肝细胞肝癌的表达及与临床病理参数的关系。 方法 采用免疫组化ABC法检测syndecan 1在肝细胞肝癌中的表达。 结果 发现在 47例肝癌组织中syndecan 1阴性表达 2 8例 ,其分化不良的肝癌syndecan 1阴性表达率高于分化较好者 (分别为 78.3 % ,41.7% ,两者相比P <0 .0 5 ) ;直径 >5cm的肝癌syndecan 1阴性表达率高于直径≤ 5cm肝癌 (分别为 81.0 % ,42 .3 % ,两者相比P <0 .0 1) ;大体或镜下有癌栓者syndecan 1阴性表达率高于无癌栓的肝癌 (分别为 84.2 % ,42 .9% ,两者相比P <0 .0 1)。syndecan 1的阴性表达与患者血清甲胎蛋白水平及Child分级均无明显的相关性 (P >0 .0 5 )。结论 syndecan 1的低表达与肝癌增大、分化程度低、侵袭转移发生等恶性表型相关 ,可能对这些表型起负调节作用 ,可视为抑癌基因。

Objective To investigate the relationship between syndecan-1 protein and malignant phenotypes of hepatocellular carcinoma (HCC). Methods forty-seven formalin-fixed sections were obtained. The syndecan-1 was measured with immunohistochemistry assay (ABC method). Results Among 47 HCC tissues, 28 (59.6%) show negative staining, the syndecan-1 negative rate in HCC with poor differentiation was higher than those with good differentiation (78.3% vs 41.7%, P<0.05), and negative rate in large HCC was higher than in small HCC (81.0% vs 42.3%, P< 0.01), and negative rate in HCC with the presence of tumor-cells in blood was greater than in those without tumor-cells in blood (84.2% vs 42.9%, P<0.01). No correlation was found between syndecan-1 expression and serum AFP level and Child class. Conclusion Syndecan-1 expression is correlated with the growth, differentiation, invasiveness, metastasis and progression of HCC. It is possible that syndecan-1 is a negative regulator of these malignant phenotypes of HCC, can be regarded as suppressive gene.