肝细胞癌中CD_(15)和nm23H1的mRNA表达及其意义

EXPRESSION OF mRNA OF CD_(15) AND nm23H1 IN HEPATOCELLULAR CARCINOMA AND THEIR SIGNIFICANCE

目的 研究CD15mRNA表达与肝细胞癌 (HCC)侵袭转移和预后关系 ,以及与nm2 3H 1mRNA表达的相关性。方法 应用原位杂交和免疫组织化学方法 ,检测分析HCC组织中CD15mRNA及其蛋白和nm 2 3H1mRNA表达。结果 99例HCC中 ,CD15mRNA及其蛋白和nm2 3H1mRNA表达阳性率分别为 3 8.4%、3 6.4%和 76.8%。CD15mRNA表达与蛋白表达一致 ,与nm2 3H1mRNA表达呈负相关。CD15mRNA及其蛋白和nm2 3H1mRNA表达均与HCC侵袭转移倾向和患者预后相关。结论 检测CD15表达有可能成为HCC侵袭转移和预后判断的一项新的病理生物学指标。

Objective To investigate the association of the expression of CD 15 mRNA with the invasion and prognosis of hepatocellular carcinoma (HCC) and the expression of nm23H1 mRNA. Methods In situ hybridization and immunohistochemistry methods were used to detect the expression of CD 15 mRNA and protein nm23H1 mRNA in HCC. Results In 99 cases of HCC, the positive rate of CD 15 mRNA,its protein and nm23H1 mRNA were 38.4%, 36.4% and 76.8%, respectively. The expression of CD 15 mRNA was consistent with its protein and negatively correlated with the expression of nm23H1 mRNA. The expression of CD 15 mRNA and its protein, nm23H1 mRNA were associated with the invasiveness and metastasis of HCC and the prognosis of HCC patients. Conclusion The detection of CD 15 expression could be a new pathological biology index to judge the metastasis and prognosis of HCC.