银强化斑点金免疫渗滤法检测微量结核杆菌膜抗原

Application of silver enhancement dot immunogold filtration assay to test trace mycobacterium tuberculosis antigen.

目的 采用免疫金银染色法 (IGSS)增强斑点金免疫渗滤法 (DIGFA)的敏感性 ,用于检测微量样本。方法 将IGSS与DIGFA相结合 ,并设计一简易渗滤装置 ,形成银强化斑点金免疫渗滤法 (SEDIGFA)。结果 以测定微量结核杆菌膜抗原为模式进行上述银加强试验 ,SEDIGFA较DIGFA敏感性增强。结论 SEDIGFA可增强微量样本的敏感性 ,且便于实验室制作和推广应用。

To imprive the sensitivity of dot immumogold filtration assay(DIGFA) to test trace mycobacterium tuberculosis antigen. Methods We studied on silver enhanced DIGFA (SEDIGFA) and immunogold silver staining. Results SEDIGFA was more sensitive than DIGFA. Conclusion SEDGFA can be used to test trace samples.