蜂毒素体外抑瘤作用的实验研究

The anti-tumor effect of melittin in vitro

目的 :研究蜂毒素对建系肿瘤细胞体外生长的抑制作用。 方法 :通过凝胶色谱法从蜜蜂毒中纯化得高纯度蜂毒素 ,分别选取 SMMC- 772 1、BEL - 740 2和 Hep- 3B三种肿瘤细胞系 ,以四甲基偶氮唑盐 (MTT)比色法观察蜂毒素对 3种肿瘤细胞系的生长抑制作用 ,以丝裂霉素、长春新碱、华蟾素为对照 ;并考察了蜂毒素对 SMMC- 772 1细胞系的生长抑制作用的时效关系。结果 :蜂毒素的抑瘤作用与剂量呈正相关 ,抑制率优于对照组药物 ,蜂毒素对 SMMC- 772 1细胞系各时间点的抑瘤率无显著性差异。 结论 :蜂毒素的体外抑瘤作用明显 ,而且作用发生迅速。

Objective: [WT5”,6BZ]To investigate the anti tumor effects of melittin in vitro . Methods: Melittin was isolated and purified from bee venom by gel filtration. The cytotoxicity of melittin against established cell lines(SMMC 7721,BEL 7402 and Hep 3B cell) was determined using by MTT assays. Mitomycin, vincristine and cinobu facini were used as control. The time effect relationship of melittin against SMMC 7721 cell line was studied. Results: Melittin showed the anti tumor effect in a dose dependent matter for tumor cells in vitro ,but there was no time effect relationship. The anti tumor effect of melittin was better than the control. Conclusion: Melittin has marked and quick anti tumor activity in vitro against established cell lines. [