摘要
目的 :研究 Kkay 型糖尿病小鼠与正常小鼠肝脏差异表达的基因 ,为人类 型糖尿病及其慢性并发症的防治提供依据。 方法 :以包含 8192条小鼠的 c DNA基因表达谱芯片研究 Kkay 型糖尿病小鼠肝脏的基因表达谱。 结果 :共筛选出差异表达基因 15 4条 ,包括全长基因 6 8条 ,表达序列标识 (EST) 86条 ;其中 40条基因表达增加 ,114条表达降低。结论 : 型糖尿病发病机制复杂 ,不仅有糖、脂肪及蛋白质等物质的代谢紊乱 。
Objective: To investigate the genes differently expressed in the livers of Kkay diabetic and normal mice, providing data to prevent human from diabetes and its chronic complications. Methods: cDNA microarray chips containing 8 192 cDNAs were used to investigate the gene expression pattern of the liver in Kkay mouse. Results: One hundred and fifty four genes were screened out, comprising 68 complete cDNAs and expressed sequence tags, among them 40 genes were up regulated and 114 genes were down regulated. Conclusion: The pathogenesis of type Ⅱ diabetes is complicated, including the disorder of the metabolism of carbohydrate, fat and protein, and many functional abnormalities of a number of vital proteins. [
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2001年第7期631-635,共5页
Academic Journal of Second Military Medical University
关键词
基因芯片
基因表达
糖尿病
非胰岛素依赖型
病因学
gene chip
gene expression
diabetes mellitus, non insulin dependent
Kkay mouse
