碱性成纤维细胞生长因子对胎盘屏障通透性的实验研究

An experimental study on permeability of basic fibroblast growth factor through placental barrier in rats

目的 :探讨碱性成纤维细胞生长因子 (b FGF)能否通过胎盘屏障而对胎儿发挥其神经营养作用。方法 :怀孕 16 d(孕中期 ) Wistar孕鼠 ,用 1 2 5 I标记的 b FGF(1 2 5 I- b FGF)经腹腔注射 ,30 min后取孕鼠血清及胎鼠脑、心、肝、脾、肺 ,检测和比较各组织 b FGF的放射活性 ,并以同一注射剂量的 1 2 5 I- b FGF在肝脏的分布含量为参照 ,比较胎鼠脑、新生鼠脑和成年鼠脑与肝脏含量比。 结果 :(1)胎鼠体内有 1 2 5 I- b FGF的分布 ,且随外源性 1 2 5 I- b FGF注射剂量增加而升高。 (2 )在安全剂量范围 (5～ 5 0 0ng/ g)内 ,外源 b FGF剂量增加到一定值 (15 0 ng/ g)时 ,胎盘屏障通透性明显提高。 (3)注射相同剂量 1 2 5 I- b FGF,与胎鼠其他器官相比 ,胎鼠脑组织内 1 2 5 I- b FGF含量最少。(4)以肝组织 1 2 5 I- b FGF含量作参照 ,与新生鼠脑及成年鼠脑相比 ,外源性 1 2 5 I- b FGF在胎鼠脑含量最高。 结论 :b FGF能够部分通过胎盘屏障进入胎脑。

Objective: To investigate if bFGF can penetrate placental barrier. Methods: Sixteen day pregnant Wistar rats were selected. bFGF labeled with 125 I was injected peritoneally into the rats. The radioactivity of bFGF in different organs were determined in 30 min. Results: (1) 125 I bFGF was detected in the brain, heart, liver,lung and spleen. (2)With the same dose of 125 I bFGF, the concentration of it in the brain was at lowest level of all other organs.(3) In the range of safe dose, the permeability of bFGF through placental barrier was increased obviously. Conclusion: bFGF may penetrate placental barrier into rat's brain, which makes possible for the therapeutic intervention of bFGF in feotus. [