蕈样肉芽肿DNA含量流式细胞术测定及其临床意义

Study on nuclear DNA content of granuloma fungoides

目的 探讨蕈样肉芽肿 (MF)组织的DNA含量及细胞增殖水平与临床特征及预后的关系。方法 应用流式细胞术测定 6 2例石蜡包埋的MF组织中DNA含量及细胞增殖水平。结果 红斑期、斑块期、肿瘤期MF患者 ,其DNA异倍体率及DNA指数 (DI)随病情的延长而增高 ,红斑期与肿瘤期之间差异有显著性 (P <0 0 5 ) ;同样 ,肿瘤期患者组织中S期细胞比率 (SPF)及增殖指数 (PI)均明显高于红斑期患者 (P <0 0 5 )。结论 DNA异倍体及DI、SPF、PI值的高低与蕈样肉芽肿分期为有关 。

Objective To investigate the relationship among DNA content, cell proliferation with the clinical features and prognosis of granulona fungoides(MF) Methods DNA content and level of cell proliferation measured in on 62 paraffin-embedded specimens of MF using flow cytometry Results Both the aneuploidy rate and DNA index (DI) were higher in different stages of erythema, plaque and neoplasm of MF. The difference between the stages of erythema and neoplasm was significant( P <0 05), but also S-phase fraction (SPF) and proliferation index (PI)in the stages of neoplasm were much higher than those of erythema stage( P <0 05) Conclusion Aneuploidy rate, DI, SPF and PI are retaled to the stage of MF and were important factors of prognosis