复发性生殖器疱疹患者外周血CD3^+T细胞内细胞因子的检测

Intracellular Cytokine Detection in Peripheral Blood CD3~＋ T Cells of Patients with Recurrent Genital Herpes

目的研究 Th1/Th2型细胞因子产生在复发性生殖器疱疹（ RGH）发病机理中的作用及其相互关系。方法采用双色荧光抗体染色技术经流式细胞仪检测了 20例 RGH患者外周血 CD3＋ T细胞内 5种细胞因子染色阳性细胞百分率。结果 RGH患者 CD3＋总 T细胞百分率比正常人对照明显降低（ P< 0.05）； IL-2、 IFN－γ阳性细胞百分率均显著降低（分别为 P< 0.02及 P< 0.001）； IL-10、 IL-12阳性细胞则明显升高（均为 P< 0.01）； TNF－α阳性细胞则无明显改变（ P >0.05）。结论 RGH患者存在着因 Th1与 Th2细胞间的交互异常调节所致的有关细胞因子产生失衡，表现为 Th2型细胞因子（ IL-10）产生过多，而 Th1型细胞因子（ IL-2、 IFN－γ）产生过少，由此所引起的一系列细胞免疫反应抑制效应在 RGH发病机理中起着重要作用。

Objective To study the role of Th1/Th2 cytokine profile in the pathogenesis of recurrent genital herpes (RGH), and to find out the relationship between Th1/Th2 cytokines. Methods A two-colour immunofluorescent staining of cell surface antigen and intracellular cytokines (IL-2、 IL-10、 IL-12、 IFN－γ and TNF－α ) in CD3＋ T-lymphocytes of 20 patients with RGH with flow cytometric analysis were performed. Results Compared to controls, patients with RGH showed a decreased number of CD3＋ T cells（ P< 0.05） , and a decreased number of IL-2-producing and IFN－γ-producing T cells（ P< 0.02 and P< 0.001, respectively） , whereas an increased number of IL-10-producing and IL-12-producing T cells（ P< 0.01). There was no significant difference in the number of TNF－α-producing cells between the patients and controls（ P>0.05） . Conclusions RGH patients seem to have a predominance of Th2 cytokine profile, which may play an important role in the pathogenesis of RGH.