豚鼠生殖器疱疹模型及细胞培养法观察中药抗病毒胶囊的抗病毒作用

Antiviral Effect of Antivirus Capsule Containing Chinese Medicinal Herbs on Guinea Pig Model of Genital Herpes and Cell Culture

目的探讨抗病毒胶囊治疗生殖器疱疹的作用机理。方法用雌性豚鼠生殖道单纯疱疹病毒（ HSV） 2型感染的模型，采取抗病毒胶囊内服，观察豚鼠阴道内病毒滴度、外阴症状；用体外细胞培养法观察抗病毒胶囊对 HSV-2的直接灭活和三种给药途径下对 HSV-2的抑制作用。结果抗病毒胶囊组豚鼠阴道内病毒滴度与阿昔洛韦组比较差异无显著性（ P >0.05）；外阴症状评分在第 2、 3、 5、 7、 8天显著低于生理盐水对照组（ P< 0.05），与阿昔洛韦组大致相同（ P >0.05）；细胞培养结果：抗病毒胶囊 1号、 2号对 HSV-2的最低有效抑制浓度分别 0.390625 mg/mL和 1.5625 mg/mL。结论中药抗病毒胶囊在动物体内和体外对 HSV-2均有抑制作用。

Objective To study the therapeutic mechanism of the antivirus capsule containing Chinese herbs against genital herpes. Methods The female guinea pig GH models were treated with antivirus capsule. The vaginal titer of the virus and the symptoms of vulva were observed. Cell culture was used to detect the cytopathic suppression. Results It was shown that there was no difference between antivirus capsule group and acychovir (ACV) group on the titer of virus in the vagina of guinea pig (P >0.05). The symptom score after the treatment with antivirus capsule was significantly lower than that of saline control group (P< 0.05), and similar to ACV group (P >0.05). Cell culture showed that the lowest effective concentration for suppressing HSV-2 by antiviral capsule 1 and antiviral capsule 2 was 0.390625 mg/mL or 1.5625mg/mL, respectively. Conclusion Antiviral capsule containing Chinese medicinal herbs inhibits HSV-2 in vivo and in vitro.