天然抗角蛋白自身抗体抑制角质形成细胞及鳞状细胞癌细胞增殖的作用

Anti-keratin Autoantibody Inhibits Keratinocytes Proliferation of Normal Skin and Squamous Cell Carcinoma

目的 探索天然抗角蛋白自身抗体抑制角质形成细胞及鳞状细胞癌细胞增殖的作用机理。方法 经体外培养人角质形成细胞及鳞状细胞癌细胞，作用于不同浓度的天然抗角蛋白自身抗体，四甲基偶氮唑盐法检测活性，免疫组化检测增殖及凋亡相关蛋白变化。结果 天然抗角蛋白自身抗体对二者的增殖有着明显的抑制作用，且呈一定的浓度－效应关系。对二者 P53、 C-myc的表达具有促进作用 ,而对增殖细胞核抗原的表达则具有抑制作用。结论 天然抗角蛋白自身抗体对角质形成细胞及鳞状细胞癌细胞增殖的抑制作用，可能系通过调节细胞中增殖与凋亡相关蛋白的表达来实现。

Objective To study the mechanism of anti-keratin autoantibodies (AK auto Ab) inhibiting the keratinocytes proliferation. Methods Keratinocytes from normal human skin and squamous cell carcinoma were cultured, then treated with AK auto Ab in different doses. MTT and immunohistochemical technologies were applied to test the proteins related to proliferation and apoptosis. Results AK could inhibit the proliferation of these two kinds of cells with a dose related effect. AK auto Ab could promote the expression of p53,c-myc, and inhibited the expression of PCNA. Conclusions The results indicate that AK auto Ab plays an important role in the proliferation of keratinocytes both from normal skin and from SCC.