期刊文献+

金属蛋白酶抑制剂BB-94抑制腺样囊性癌侵袭转移的实验 被引量:3

An experimental study of matrix metalloproteinase inhibitor BB-94 inhibits the invasion and metastasis of the human salivary adenoid cystic carcinoma in vitro and in vivo
原文传递
导出
摘要 目的 观察金属蛋白酶抑制剂BB 94对涎腺腺样囊性癌 (adenoidcysticcarcinoma ,ACC)细胞侵袭、转移能力的抑制作用。方法 癌细胞体外侵袭能力用重组基底膜侵袭实验测定 ,聚丙烯酰胺凝胶电泳 (polyacrylamidegelelectrophoresis,PAGE)底物酶谱法检测Ⅳ型胶原酶活性 ,用腺样囊性癌ACC M细胞系裸鼠肺转移模型观察体内抑制转移作用。结果 在 10 μmol/L和 10 0 μmol/L时 ,BB 94对ACC M细胞侵袭重组基底膜的抑制率分别为 32 9%和 5 3 2 % ;对ACC M细胞培养上清中的Ⅳ型胶原酶活性有降低作用。在裸鼠肿瘤转移实验中 ,对照组和用药组的肺转移率分别是 90 0 %和2 0 0 % ,P <0 0 1;含转移灶肺重量分别为 (0 2 0 90± 0 0 6 6 7)g和 (0 15 32± 0 0 378)g ,P <0 0 5。结论 金属蛋白酶抑制剂BB 94能明显抑制ACC M细胞侵袭重组基底膜和ACC的肺转移。 Objective To study the inhibitory effect of matrix metalloproteinase inhibitor BB-94 on t he invasion and metastasis of salivary adenoid cystic ca rcinoma in vitro and in vivo. Methods Reconstituted basement m embrane invasion assay was used to evaluate invasive ability of cancer cells. Ty pe Ⅳ collagenase was assessed by PAGE substrate zymography. In addition, the an timetastasis effec t of the compound was studied in in vivo experiments using nude mice model o f pu lmonary metastasis with ACC-M line of human salivary adenoid cystic carcinoma. Results BB-94, at the concentrations of 10μmol/L and 100 μm ol/L, suppressed the reconstituted basement invasion of ACC-M human salivary ad enoid cystic carcinoma by 329%, 532% and reduced Type Ⅳ collagenase activit ies in the serum-free supernatant of the same cells. The pulmonary metastasis r ate of the control and treated groups was 900% and 200%, respectively (P <001). The lung weight of the control and treated groups was (0209 0±0 066 7) g and (0153 2±0037 8) g , respectively (P<005). Conclusions These result s suggest that the matrix meta lloproteinase inhibitor BB-94 has a strong inhibitory effect on invasion and me tastasis of salivary adenoid cystic carcinoma.
出处 《中华口腔医学杂志》 CAS CSCD 北大核心 2001年第4期304-307,共4页 Chinese Journal of Stomatology
关键词 腺样囊性 金属蛋白酶类 组织抑制剂 肿瘤转移 ACC Carcinoma,adenoid cystic Tissue inhibitor of metalloproteinases Neoplasm metastasis
  • 引文网络
  • 相关文献

参考文献7

  • 1李红梅,中华医学杂志,1997年,77卷,652页
  • 2Eccles S A,Cancer Res,1996年,56卷,2815页
  • 3Watson S A,Cancer Res,1995年,55卷,3629页
  • 4Sledge G W,J Natl Cancer Inst,1995年,87卷,1546页
  • 5Wang X,Cancer Res,1994年,54卷,4726页
  • 6Aznavoorian S,Cancer,1993年,1368页
  • 7Davies B,Cancer Res,1993年,53卷,2087页

同被引文献23

  • 1侯振江,张宗英.基质金属蛋白酶类测定的临床应用[J].中国实验诊断学,2006,10(2):216-218. 被引量:8
  • 2王晓光,罗荣城.抗肿瘤新生血管靶向治疗进展[J].临床肿瘤学杂志,2006,11(2):155-158. 被引量:7
  • 3万进,吴泽宇,林华欢,骆新兰,张威,杜嘉林,姚远,王志度.中下段直肠癌基质金属蛋白酶-2表达与直肠系膜转移的关系[J].中华胃肠外科杂志,2006,9(2):136-138. 被引量:2
  • 4刘岳强,刘开泰,杨晓燕,钟近洁,许永华,张东辉,周婷婷.乳鼠成骨细胞体外培养[J].中国实验动物学报,2007,15(3):231-233. 被引量:4
  • 5Ridley AJ, Schwartz MA, Burridge K, et al. Cell migration: integrating signals from front to back [ J]. Science, 2003, 302 : 1704.
  • 6Koistinen P, Ahonen M, Kahari VM, et al. alphaV integrin promotes in vitro and in vivo survival of ceils in metastatic melanoma [J]. Int J Cancer, 2004, 112:61 -70.
  • 7Nikkola J, Vihinen P, Vlaykova T, et al. Integrin chains betal and alphav as prognostic factors in human metastatic melanoma [ J ]. Melanoma Res, 2004, 14:29 - 37.
  • 8Yang Y, Dang D, Atakilit A, et al. Specific alpha v integrin receptors modulate K1735 murine melanoma cell behavior [ J ]. Biochem Biophys Res Commun, 2003, 308:814- 819.
  • 9Felding-Habermann B, Fransvea E, O'Toole TE, et al. Involvement of tumor cell integrin alpha v beta 3 in hematogenous metastasis of human melanoma cells [ J ]. Clin Exp Metastasis, 2002, 19:427 - 436.
  • 10Zhu Z, Bohlen P, Witte L. Clinical development of angiogenesis inhibitors to vascular endothelial growth factor and its receptors as cancer therapeutics[J]. Curr Cancer Drug Targets, 2002, 2(2) : 135 156

引证文献3

二级引证文献13

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部